The anti-nociceptive effect of BPC-157 on the incisional pain model in rats
정영훈, 김해규, 김혜진, 김은수, 백지석, 강현종,
소속 상세정보
정영훈 ( Jung Young-Hoon ) - Pusan National University Hospital Departments of Anesthesia and Pain Medicine
김해규 ( Kim Hae-Kyu ) - Pusan National University Hospital Departments of Anesthesia and Pain Medicine
김혜진 ( Kim Hyae-Jin ) - Pusan National University Hospital Departments of Anesthesia and Pain Medicine
김은수 ( Kim Eun-Soo ) - Pusan National University Hospital Departments of Anesthesia and Pain Medicine
백지석 ( Baik Ji-Seok ) - Pusan National University Hospital Departments of Anesthesia and Pain Medicine
강현종 ( Kang Hyun-Jong ) - Pusan National University Hospital Departments of Anesthesia and Pain Medicine
Abstract
Background: The pentadecapeptide BPC-157 has been shown to have anti-inflammatory and wound healing effects on multiple target tissues and organs. Peptides have potent anti-inflammatory effects on periodontal tissues in rats with periodontitis. Few studies have investigated the effect of BPC-157 on pain after dental procedures or oral surgeries. The purpose of the present study was to investigate the antinociceptive effects of BPC-157 on postoperative incisional pain in rats.
Methods: Sprague?Dawley rats were randomly divided into five groups: control (saline with the same volume), BPC10 (10 μg/kg of BPC-157), BPC20 (20 μg/kg of BPC-157), BPC40 (40 μg/kg of BPC-157), and morphine (5 mg/kg of morphine). A 1-cm longitudinal incision was made through the skin, fascia, and muscle of the plantar aspect of the hind paw in isoflurane-anesthetised rats. Withdrawal responses were measured using von Frey filaments at 0, 2, 6 h and 4, 7 d after incision. The formalin test was also performed to differentiate its anti-nociceptive effect from an inflammatory reaction or central sensitization. Pain behavior was quantified periodically in phases 1 and 2 by counting the number of flinches in the ipsilateral paw after injection with 30 μL of 5% formalin.
Results: The threshold of mechanical allodynia was significantly increased in the BPC10, BPC20, BPC40 and morphine groups compared with that in the control group at 2 h. These increasing thresholds then returned to the levels of the control group. The BPC-157 group showed a much higher threshold at 4 days after incision than the control group. The thresholds of the BPC groups, except the morphine group, were normalized 7 days after incision. The flinching numbers of the BPC10, BPC20, BPC40 and morphine groups were significantly decreased in phase 1, but there was no decrease in the BPC-157 groups except the morphine group in phase 2.
Conclusions: BPC-157 was effective only for a short period after incision. It was also effective during phase 1 but not during phase 2, as determined by the formalin test. BPC-157 might have a short antinociceptive effect, even though it has anti-inflammatory and wound healing effects.
키워드
BPC-157; Formalin Test; Incisional Pain Model; Mechanical Allodynia
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